Signal Transduction — Single Cell Techniques

Signal Transduction — Single Cell Techniques

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A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.This laboratory manual provides not only insight into a variety of powerful single cell techniques, but also gives ... and practical background of the patch-clamp technique using electrical simulation circuits (without the necessity of having aanbsp;...

Title:Signal Transduction — Single Cell Techniques
Author: Bert, van Duijn, Anneke Wiltink
Publisher:Springer Science & Business Media - 2012-12-06

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